signal-processing software lab chart 7.2 Search Results


90
Cell Signaling Technology Inc annexin v–pi staining
(A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.
Annexin V–Pi Staining, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc simplechip kit
(A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.
Simplechip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/simplechip kit/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
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MathWorks Inc r2016b
(A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.
R2016b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc atp biosynthetic process
(A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.
Atp Biosynthetic Process, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Malvern Panalytical 7032n=72c multi-8-bit 72-channel digital correlator
(A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.
7032n=72c Multi 8 Bit 72 Channel Digital Correlator, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.

Journal: Life Science Alliance

Article Title: Trans-differentiation of trophoblast stem cells: implications in placental biology

doi: 10.26508/lsa.202201583

Figure Lengend Snippet: (A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.

Article Snippet: After 48 and 72 h, co-cultured cells were processed either for both annexin V–PI staining using early apoptosis detection kit (cat no. 6592; Cell Signaling Technology) by flow cytometry or for protein isolation and Western blotting for detection of apoptosis markers.

Techniques: Cell Culture, Western Blot, Software