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Image Search Results
Journal: Life Science Alliance
Article Title: Trans-differentiation of trophoblast stem cells: implications in placental biology
doi: 10.26508/lsa.202201583
Figure Lengend Snippet: (A) Apoptotic death induced by increasing dose of TRAIL in endothelial cells (MS1) and differentiated trophoblast cells (TC) assessed using annexin V–PI–based flow cytometric analysis. Corresponding untreated cells were kept as controls. (A, B, C) Quantitative analysis of percentage of from (A) in MS1 cells and (C) TC has been shown in bar graphs. Data are representative of three independent biological replicates. Error bar represents SEM. *** P < 0.001; ns, nonsignificant. (D, E) Annexin V–PI–based flow cytometric analysis to assess apoptotic death of endothelial cells (MS1) co-cultured either in the absence or presence of differentiated trophoblast cells for 48 h (D) or 72 h (E). Quantitative analysis of percentage of cells undergoing apoptotic death has been shown in adjacent bar graphs. (F) Western blot analysis of the TNFSF10 (TRAIL) agonistic receptor DR4 using cell lysate from MS1 co-cultured either in the absence (control) or presence of differentiated trophoblast cells and cell lysate from co-cultured trophoblast cells (TC). (F, G) Densitometric analysis of the proteins from (F) using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. (H, J) Western blot analysis of proteins from extrinsic (H) and intrinsic (J) apoptotic pathway proteins using lysates from MS1 cells co-cultured in the absence or presence of TC. (G, I, K) Densitometric analysis of the proteins from (G, I), respectively, using NIH ImageJ software after normalization with GAPDH. Data are representative of three independent biological replicates. Error bars represent SEM. ** P < 0.01; ns, nonsignificant. Source data are available for this figure.
Article Snippet: After 48 and 72 h, co-cultured cells were processed either for both
Techniques: Cell Culture, Western Blot, Software